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In vitro Studies and Antimicrobial Activities of Pereskia grandifolia Haworth var. grandifolia

By walaa on June 15,2008

 

Rosna Mat Taha* and Fatimah Abdol Latif 

ABSTRACT

       In vitro propagation was developed for the medicinal plant Pereskia grandifolia Haworth var. grandifoliausing axillary bud explants. Optimum multiplication of shoots was achieved on MS (Murashige andSkoog, 1962) medium supplemented with 3.0 mg/l BAP (6-Benzylaminopurine) and 30.0 mg/l adenine.Plants were maintained in vitro on MS medium while callus were induced on MS basal mediumsupplemented with the combination of 5.0 mg/l BAP (6-Benzylaminopurine) and 5.0 mg/l NAA (α-Naphthalene acetic acid). The antimicrobial tests were carried out using the crude extracts of this speciesfrom in vitro callus and intact leaves. No difference was detected between crude methanol extract fromPereskia grandifolia leaves and callus. The same extract failed to inhibit all gram positive and gramnegative bacteria growth; however, it inhibited the growth of fungi. Only dermatophytes fungi (withoutspore) such as Microsporium canis and Trichophyton rubrum was inhibited with a medium and weakstrength of methanol extract. This extract did not inhibit the growth of dermatophytes fungi (with spore)such as Aspergillus niger and Trichophyton mentagrophyte.

Keywords: Pereskia grandifolia, Microsporium canis, Trichophyton rubru, adenine, BAP, NAA, callus.



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