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Long-term Stability of Two Rauwolfia serpentina Cell Strains

By walaa on June 23,2008


 Igor O. Andreev, Daniel Adnof, Katerina V. Spiridonova, and Viktor A. Kunakh  


      In these latter days there is ever growing demand for medicinal drugs and nutrient supplements of natural origin, in particular derived from plant raw material. At the same time, exhaustion of natural resources has risen the problem of search for new alternative sources of plant raw material. Cell biotechnology may propose the way out of this situation. Development of the techniques for maintenance of the plant cells, tissues and organs under controlled conditions on the artificial nutrient media provides the possibility for production of desirable amounts of ecologically clean plant biomass. In this work we investigated the long-term genetic stability of plant tissue culture, which is prerequisite for its durable use for industrial production of plant based drugs. Genetic analysis of Rauwolfia serpentina Benth. cultured tissues from two strains to be sampled with an interval of time more than 10 years was carried out through RAPD-PCR. The genome of cultured plant cells was found to lack of obvious changes during long-term maintenance. The data obtained evidence for significant stability of the tissue culture under growth in standard condition. Used RAPD-markers seem to allow differentiate various strains of cultured R. serpentina tissues.Keywords: Rauwolfia serpentina, plant tissue culture, in vitro, cell line K-20, cell line K-27, indoline alkaloids production, ajmaline, genetic polymorphism, RAPD-PCR, plant raw material.

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