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In vitro Formation of Synthetic Seed from Microshoots of Begonia x hiemalis Fotch.

By walaa on June 23,2008

 

 Asmah Awal, Rosna M. Taha and Nor A. Hasbullah 

Abstract

    Artificial seed or synthetic seed formation of Begonia x hiemalis Fotch var. Schwabenland Red had been induced from leaf explants in vitro. The explants were cultured on (Murashige and Skoog (MS) medium supplemented with combinations of 1.0 mg/l Benzylamino purine (BAP) and 1.0 mg/l Naphthalene acetic acid (NAA). After 8 weeks, regenerated microshoots were excised from leaf cultures and microshoots successfully encapsulated in 3% sodium alginate solution together with MS salt solution and polymerized within 1.0M CaCl2.2H2O solution for 30 minutes. The beads produced were with firm coats, round beads and uniform size and in good shape for handling.  The results demonstrate that the optimum germination and survival rate of encapsulated matrix was in MS medium containing 3% sodium alginate solution supplemented with 3% sucrose, 1.0 mg/l BAP in combination with 1.0 mg/l NAA. The viability of the encapsulated micro shoots after storage period at 4 °C was also determined. High germination rate (100%) was achieved after 1-3 months storage whereas low germination rate (7-53%) was obtained after 4-6 months storage. The seeds were also successfully germinated in three different germination media including MS basal, garden soil and vermiculite.Keywords: Begonia x hiemalis Fotch., encapsulated microshoots, synthetic seeds, tissue culture.


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