Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System

 

Hamdy Atta-Alla1*, Mostafa Zaghloul1, Abd El Kawee Waly1, Matthias Fladung2, and Fadia El-Sherif1

1Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

2Johann Heinrich von Thuenen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries,

Institute for Forest Genetics, Sieker Landstr. 2, D-22927 Grosshansdorf, Germany

ABSTRACT

Based on the Ac/Ds two element transposition system from maize an activation tagging approach was

suggested for the hybrid aspen (Populus tremula x tremuloides) line ‘Esch5’. A glucocorticoidinducible

two element Ac/ATDs element system was used to induce activation tagged variants

following two independent transformation steps. In combination with a 35S enhancer tetramer and

outward facing two CaMV 35S promoter located near both ends of the ATDs element, expression of

genes can be elevated located adjacent to the new integration site of the element. As selective marker

for ATDs transposition, knocking-out the expression of a phenotypic marker (rolC gene) was

considered.

Keywords: Activation tagging, glucocorticoid-inducible, rolC gene, Ac/Ds, transposon, ATDs

element.