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Activation tagging in aspen using a heat-shock inducible two component Ac/Ds-enhancer element system

By Doaa Medany on August 15,2011



Hamdy Atta-Alla1, Mostafa Zaghloul1, Abd El Kawee Waly1, Matthias Fladung2, and Fadia El-Sherif1*

1Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

2Johann Heinrich von Thuenen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries,

Institute for Forest Genetics, Sieker Landstr. 2, D-22927 Grosshansdorf, Germany



From our earlier work on the genetic transfer of the maize transposable element Ac and its functional

analysis in hybrid and pure aspen lines, it was shown that this element is active in transgenic aspen.

However, a two element transposon tagging system where the Ds element and the transposase gene are

put together in crosses is not feasible in trees due to the in part long vegetative phases. To overcome

this barrier, an inducible two element HSP-transposase /ATDs element system is suggested to induce

activation tagged variants following two independent transformation steps. The ATDs element is

comprised by a 35S enhancer tetramer and outwards facing two CaMV 35S promoter located near both

ends of the ATDs element. Following transposition of the ATDs element expression of genes can be

elevated which are located adjacent to the new integration site of the element.

Keywords: Activation tagging - rolC gene - HSP-transposase - ATDs element.


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