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Micropropagation for conservation of two rare Capparis species from Egypt

By Doaa Medany on April 19,2012

 

 

Micropropagation for conservation of two rare Capparis species from Egypt


Ghada A. Hegazi1, Saad R. Eid1, Abd  El Monem M. Sharaf2

1Tissue Culture Unit, Plant Genetic Resources Department, Desert Research Center, Cairo, Egypt.

2Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.

 

 Abstract

An efficient protocol for micropropagation was developed to conserve two rare Egyptian Capparis species; Capparis orientalis Duh. and Capparis leucophylla DC., using stem node sections and shoot tips. In vitro propagation of these two species has not been previously reported. Concerning C. orientalis; Murashige and Skoog (MS) medium containing 3 mg/L benzyl adenine (BA) with or without 0.2 mg/L 2-naphthalene acetic acid (NAA) were the most suitable media for the establishment of both stem node sections and shoot tips, and MS medium containing 3 mg/L BA was optimum for the multiplication of explants. Δ²- isopentenyladenine (2iP) gave promising results in enhancing elongation of axillary shoots of C. orientalis, when added to MS medium at a concentration of 1 mg/L in addition to 3 mg/L BA.  The highest rooting percentage of C. orientalis (60%) was obtained on MS medium supplemented with 1 mg/L of both indole-3-butyric acid (IBA) and NAA after 60 days of incubation. With respect to C. leucophylla; MS medium containing 1 or 3 mg/L BA and that containing 2.5 mg/L of both BA and 2iP were the best for the establishment of stem node sections and shoot tips.  MS medium containing 1 or 3 mg/L BA were the most promising media for the proliferation of explants. Gibberellic acid (GA3) at a concentration of 3 mg/L gave the best results in enhancing elongation of axillary shoots of C. leucophylla explants. The highest rooting percentage reached only 20% and was obtained on MS medium containing 2 mg/L IBA + 0.5 mg/L NAA after 60 days of incubation. An average of 92–98% of the acclimatized transplants of both C. orientalis and C. leucophylla survived after transferring into peatmoss:sand mixture (1:1 v/v) in the greenhouse conditions.

Key words: Capparis orientalis, Capparis leucophylla, in vitro propagation,  stem node sections, shoot tips.

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