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Effect of Carbon Sources and Microelement Concentrations on in vitro Proliferation and Rooting of Pineapple (Ananas comosus)

By walaa on June 15,2008

 

Nagwa S. Zaied 

Abstract

        The regenerated shoots of Pineapple (Ananas comosus Cv. Smooth Coyenne) during the establishmentstage were cultured individually on MS medium supplemented with BAP (2.0 mg/L) during theproliferation stage and IBA (2.0 mg) during the rooting stage. Various sugars (glucose, fructose andsucrose) and concentrations (0, 10, 20 and 30 mg/L) were tested. Copper and Boron microelementsconcentrations were also tested. Data indicated that all sucrose treatments enhanced the proliferation androoting, however Glucose and fructose improved shoot length. Increasing copper sulfate to (0.05 mg/L)produced the best proliferation. Meanwhile, Boric acid at the rate of 12.4 mg/L was the most suitableconcentration for improving rooting percentage.

Keywords: Ananas cosus, carbon source, microelements, proliferation.



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