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Micropropagation of Pelargonium sidoides

By walaa on June 15,2008

 

 Francis B. Lewu, Don S. Grierson and Anthony J. Afolayan

Abstract

      Pelargonium sidoides is a medicinal plant harvested for local and export trades in South Africa. In orderto contribute to the conservation and propagation initiatives of the herb, a study on the micropropagationof the plant was conducted. Our findings revealed that callus induction was achieved by using differentconcentrations of IBA and 2,4-D. Optimal response was obtained at 3 mg l-1 IBA under continuous darkcondition. The study also showed that shoot differentiation is achievable from stem derived callus afterone week in hormone free media under continuous light. The percentage of callus formation ranged from0 to 88.9% depending on the type of auxin used, the concentration and photogenic conditions.Transferred plantlets by simple acclimatization procedure survived and produced healthy plants. Thesignificance of these on the propagation and conservation of P. sidoides is highlighted in the discussion.Keywords: Callus induction; Eastern Cape; ex situ conservation; growth regulators; medicinal plants;

micropropagation; Pelargonium sidoides; South Africa.



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